Microchip-Based Assay Systems: Methods and Applications (Methods in Molecular Biology)

This volume explores recent progress in the microelectronics arena, the
resultant miniaturization of component device features to nanometer
size particles, and the ensuing growth in the development and use of
microchip-based techniques in leading laboratories around the world.
While the area of microelectronics is faced with daunting challenges,
it promises fascinating new advances that may translate into products
such as sensors and devices that use nanometer-sized building blocks
for real-world applications. The contributors to this volume are part
of growing interdisciplinary efforts among pioneers in
microelectronics, nanoscience, and health that will continue to create
exciting new microchip-based applications across a variety of sectors.
In the 16 chapters of this volume, leading scientists present
technically precise microchip-based assays for a variety of
applications.

Highlights include: methods for microchip electrophoresis for DNA
separation; fabrication of porous polymer monoliths in microfluidic
chips for selective nucleic acid concentration and purification; rapid
electrical lysis of bacterial cells in a microfluidic device; an
on-chip bioassay that uses immobilized sensing bacteria in a
three-dimensional microfluidic network; and impedimetric detection for
DNA hybridization within microfluidic biochips. Molecular diagnostic
and immunologic protocols include microchip-based enumeration of human
white blood cells; microchip diagnosis of cervical cancer; protocols
for DNA microchips toward molecular signatures in cervical cancer; a
microchip based assay for Interleukin-6; and methods for
microchip-based electrochemical enzyme immunoassays. Several chapters
are devoted to topics in microarray technology, including: applications
of functional protein microarrays; allergen microarrays for the
diagnosis of specific IgE in a multiplex biochip-based immunoassay;
protein-array based multiplexed cytokine assays; and lectin microarrays
for glycoprotein assays.