Alongside developing new ones, the optimization of existing methods is a key task in the HPLC laboratory. And a task that nowadays has to be solved increasingly fast and cost-efficiently.This handbook provides well-founded assistance in better meeting this challenge. Internationally renowned authors cover both the general basics and strategies in optimization, as well as the specific aspects involved in such different methods as RP-HPLC, NP-HPLC, micro- and nano-HPLC, and hyphenated techniques, such as LC/MS. Nor are such topics as column selection and chiral separations left uncovered. Some of the contributions present applications using common optimization software, such as DryLab or ChromSword. All of the chapters concentrate on the essentials and are written in a practically oriented style, while their self-contained structure allows for targeted references.Throughout the text, the authors offer concrete, practical tips as well as pertinent background information, together with insights into the optimization methods of seven major international companies from various sectors.The whole is rounded off with real-life reports from experienced users coming from the different areas of application, in particular the life sciences, such as proteomics or drug development.

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