This book constitutes the refereed proceedings of the International Conference on Mass Data Analysis of Signals and Images in Medicine, Biotechnology and Chemistry, MDA 2006 and 2007, held in Leipzig, Germany..The topics include techniques and developments of signal and image producing procedures, object matching and object tracking in microscopic and video microscopic images, 1D, 2D and 3D shape analysis, description and feature extraction of texture, structure and location, image segmentation algorithms, parallelization of image analysis and semantic tagging of images from life science applications.