A timely collection of current and state-of-the-art techniques for protein and peptide preparation and sequencing-all described by hands-on, masters of the procedures. Conveys valuable lessons and insights into using automated sequencers and mass spectrometers, preparing proteins and peptides on a microgram and submicrogram scale, mapping peptides, analyzing amino acids by pre- and post-column derivatization methods, and sequencing N- and C-terminal peptides by classical and newly developed techniques. Also deals with such troublesome problems as identifying sites of modification and working with very small samples.

Скачать книгу бесплатно (pdf, 19.66 Mb)

---

Читать «Protein Sequencing Protocols »