The critically acclaimed laboratory standard for more than forty years, Methods in Enzymology is one of the most highly respected publications in the field of biochemistry. Since 1955, each volume has been eagerly awaited, frequently consulted, and praised by researchers and reviewers alike. More than 270 volumes have been published (all of them still in print) and much of the material is relevant even today - truly an essential publication for researchers in all fields of life sciences.Key Features* Promoter elements and RNA polymerase components* RNA polymerase and its subunits in prokaryotes* Biochemical assays of transcription initiation* RNA polymerase and associated factors from eukaryotes* Genetic analysis of transcription and its regulation